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ToxicodynamicsDEB theory captures effects of chemicals on energetics via changes in parameter values, linked to internal concentrations. All DEB parameters are potentially affected. The hazard rate, also called instantaneous death rate, classifies as state (i.e. a function of other states and parameters) that is a target for aging and starvation, for instance. Lethal effects are captured via effects on the hazard rate; other effects are called sublethal effects. DEB parameters have been chosen such that each of them relates to a single metabolic flux, one parameter per flux, exposing the potential handles for chemicals to modify individual performance Three concentration ranges are delineated for each parameter: "too little", "enough" and "too much". Within the "enough"-range for a particular parameter, the parameter does not depend on the concentration. The boundaries of this parameter-specific range are called the (lower and upper) internal no-effect concentrations. Changes in parameter values are proportional to the difference between the internal concentration and the (relevant) no-effect concentration for small changes in parameter values, on the basis of Taylor's approximation. Small changes in parameter values can, however, result in large changes in measured quantities (i.e. growth or reproduction), which are called endpoints. Effects on several endpoints might be due to a change in single parameter. Toxicants are chemicals for which the "too little"-range is zero, i.e. the lower no-effect concentration is zero; the upper no-effect concentration then becomes THE no-effect concentration for that parameter. For some compounds, such as most mutagenic compounds, even the no-effect concentration is zero and all internal molecules have effects. For increasing internal concentrations of toxicants, first one, then more and more parameters become affected. If the concentration range for which a single parameter is affected, is rather large, this parameter represents the effective mode of action of the toxicant (in low concentrations). Effects on a single parameter can translate into effects on multiple endpoints. Toxicants with a similar biochemical mode of action, can have different effective modes of action and vice versa.
Cases where just a single toxicant is involved are rare, due to chemical and metabolic transformation, e.g. ionization. Lipophilic compounds are frequently transformed to more hydrophilic (and more toxic) ones, enhancing elimination. Toxicity studies are typically motivated by potential pollution or exposure scenarios, which usually involve mixtures directly. Theory has been developed for how mixtures of chemical compounds affect parameters. DEB theory also has modules for the growth of tumors in relation to the state of the individual, including the nutritional condition.
ToxicokineticsThe basic toxicokinetic model is the one-compartment model, where uptake is proportional to the external concentration and elimination is proportional to the internal concentration. In a DEB context, these rates are also proportional to surface area, which links them to structural length of individuals; small individuals more rapidly equilibrate with the (changing) chemical environment, compared to bigger ones. Dilution by growth is easy to account for in a DEB context; even little growth can affect internal concentration-profiles substantially. Although this simple one-compartment model frequently captures the broad picture well, in practice deviations occur, for which variants of the basic model have been developed.
The most important variants of one-compartment models delineate several uptake and elimination routes. They account for uptake via food (especially for poorly soluble compounds), or directly from the environment (respiration, water for aquatic organisms, skin-contact for terrestrial ones), and elimination via feces, respiration, reproduction. Uptake can depend on nutritional condition via re-partitioning of the internal compound between reserve and structure (multi-compartment models), which helps to understand that effects of some toxicants only show up during starvation. Elimination is frequently an active metabolic process, involving chemical transformations. DEB theory specifies the network of interlinked metabolic processes, so concerning not only toxicodynamic, but also toxicokinetics processes.